Drug Discovery World

FEBRUARY 8, 2024

This is largely due to their highly stable Inverted Terminal Repeat (ITR) regions, as this secondary structure is prone to deletions during propagation in bacteria and cannot be read by standard Sanger sequencing. Dr O’Hara also provided an AAV plasmid preparation case study. Why are those important?

Gene Therapy 59

Gene Therapy Gene Bioinformatics Life Science 59

Drug Discovery World

OCTOBER 3, 2023

This is largely due to their highly stable Inverted Terminal Repeat (ITR) regions, as this secondary structure is prone to deletions during propagation in bacteria and cannot be read by standard Sanger sequencing. Dr O’Hara also provided an AAV plasmid preparation case study. Why are those important?

Gene Therapy 52

Gene Therapy Gene Life Science Bioinformatics 52

Drug Discovery World

APRIL 25, 2024

For instance, bacteria and yeast cell lines are easier to cultivate compared with mammalian cell lines, requiring less maintenance for the medium they are grown in. Recent years have also seen the development of technologies that enable a better genomic and transcriptomic analysis of single cells. Bacterial cell lines such as E.

Development 59

Development Protein In-Vivo Life Science 59

Drug Discovery World

AUGUST 10, 2022

The immune system typically identifies foreign entities such as bacteria and viruses and activates lymphocytes such as T cells to destroy them with overwhelming force. If the gene does not integrate into the genome, the cell will not become a CAR T cell. National Institutes of Health researcher Ping Jin, Ph.D.

Manufacturing 98

Manufacturing Gene Genome Genomics 98