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Modernizing cell culture processes for the next wave of genomic medicine

Pharmaceutical Technology

“For each production process, you have to generate the plasmids in huge quantities with GMP quality and then do a transient transfection, meaning you mix them with a transfection reagent and add that to the cells in a very controlled way,” explains Faust. Two-Plasmid Packaging System for Recombinant Adeno-Associated Virus.

Genome 244
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Optimising AAV capsid purification through improved analytics

Drug Discovery World

In AAV therapeutics, empty capsids are those that are not packaged with the therapeutic DNA. Partially-filled capsids may also be present, and contain packaged process-related impurities or truncated genetic material. Robustness: to be able to tolerate matrix effects (type of buffer, residual contaminants, ionic strength, etc.)