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Modernizing cell culture processes for the next wave of genomic medicine

Pharmaceutical Technology

The established method for this process is a triple transfection using three different DNA plasmids. Better transfection reagents and efforts to automate the currently highly manual and inconsistent process could also make a difference, he adds. For example, researchers have developed a two-plasmid cotransfection system.

Genome 244
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Optimising AAV capsid purification through improved analytics

Drug Discovery World

This helps ensure a high concentration of loaded capsids and adequate removal of impurities, such as host cell proteins and DNA. In AAV therapeutics, empty capsids are those that are not packaged with the therapeutic DNA. Robustness: to be able to tolerate matrix effects (type of buffer, residual contaminants, ionic strength, etc.)