Drug Discovery World

FEBRUARY 8, 2024

Questions were fielded from the audience, which included: What analysis will be performed to evaluate host or other DNA contamination? Dr O’Hara also provided an AAV plasmid preparation case study. What types of QC do you have for AAV synthesis and plasmid preparation? Why are those important?

Gene Therapy 59

Gene Therapy Gene Bioinformatics Life Science 59

Drug Discovery World

OCTOBER 3, 2023

Questions were fielded from the audience, which included: What analysis will be performed to evaluate host or other DNA contamination? Dr O’Hara also provided an AAV plasmid preparation case study. What types of QC do you have for AAV synthesis and plasmid preparation? Why are those important?

Gene Therapy 52

Gene Therapy Gene Life Science Bioinformatics 52

The Pharma Data

JUNE 26, 2021

It inhibits folate-dependent enzymes critical to the de-novo biosynthesis of nucleotides leading to the disruption of DNA replication. Pemetrexed is a multi-targeted antifolate anti-cancer agent that disrupts crucial folate-dependent metabolic processes essential for cell replication.

Contamination 52

Contamination DNA Production Marketing 52

Drug Discovery World

JANUARY 19, 2023

This paid-for advertorial by Catalent appeared in DDW Volume 24 – Issue 1, Winter 2022/2023 One significant challenge in the manufacturing of cell and gene therapies is the production of high-quality plasmid DNA (pDNA). coli residual contaminants as well as product-related impurities. billion by 2030 (BioSpace, 11 August 2022).

DNA 52

DNA Manufacturing Gene Therapy Gene 52

Advarra

MARCH 31, 2023

Gene Delivery Systems Genetic material, in the form of DNA or RNA, does not easily enter cells without the aid of a delivery system. These individuals provide a perspective that is equally important for consideration when evaluating the risks to the study participants and clinicians, as well as to the community where the study is conducted.

Engineer 52

Engineer Genetics Clinical Research Research 52

XTalks

NOVEMBER 7, 2022

Liquid biopsy tests in oncology involve isolating entities such as circulating tumor cells (CTC), circulating tumor DNA (ctDNA) and tumor-derived exosomes. However, Dr. Bahassi reported that the selection of CTCs using this method is often not very efficient and the CTC population is usually highly contaminated with leukocytes.

Clinical Trials 98

Clinical Trials Clinical Trials Trials FDA Approval 98

Cloudbyz

NOVEMBER 3, 2021

Before deploying collected samples for a project, small portions from those samples are retained as a precautionary measure, to account for any potential destruction or contamination of the originally designated portions of the samples. Let’s use the easiest example of a sample.

Biobanking 52

Biobanking Clinical Research Contamination Research 52

pharmaphorum

MARCH 8, 2022

DI: Oncology is a hugely exciting area at the moment, particularly the further focus on genome testing to help identify specific mutations in the DNA and select the best treatment option for the patient and their type of cancer. Darina Shopova is design engineer at Envair Technology, working on the design and development of rigid isolators.

Life Science 98

Life Science Engineer Scientist Doctors 98

Pharmaceutical Technology

FEBRUARY 3, 2023

The established method for this process is a triple transfection using three different DNA plasmids. The second, and even greater, challenge arises during the production step, where a complete set of genes — including the therapeutic gene, capsid genes, and adenoviral helper genes — are introduced into the cells. Biores Open Access.

Genome 244

Genome Genomics Medicine Gene Therapy 244

Drug Discovery World

FEBRUARY 28, 2023

At this stage, qPCR can be used to check for IVT efficiency, residual DNA contamination and adventitious agents such as microbes. The identity of the transcript is confirmed by Sanger sequencing.

Genetics 52

Genetics Genetic Analysis Vaccination Vaccine 52

Drug Discovery World

APRIL 25, 2024

Bacterial cultures can grow extremely healthy and can withstand more drastic temperatures undertaken during the transfection method – which allows plasmid DNA to enter bacterial cells. Bacterial cell lines such as E. coli have their own set of benefits however.

Development 59

Development Protein In-Vivo Life Science 59

Roots Analysis

OCTOBER 16, 2023

It involves the separation of unwanted particles from biological materials to obtain contaminant free products. Biopharmaceutical filtration systems are designed to remove impurities and contaminants, to improve safety, purity and shelf life of the product. Particle Removal: This is the primary function of these filtration systems.

Bacteria 40

Bacteria Production Contamination Protein 40

Drug Discovery World

AUGUST 10, 2022

Although this type of contamination has not yet been reported in humans, RCVs can form rapidly growing T cell neoplasms, another type of cancer. How ddPCR technology detects and quantifies DNA ddPCR technology is a highly sensitive and accurate tool used to detect and quantify known genetic sequences. Frontiers in Immunolology.

Manufacturing 98

Manufacturing Gene Genome Genomics 98

XTalks

FEBRUARY 26, 2021

It wasn’t until the early 1970s when large numbers of other cell lines were found to be contaminated with HeLa in some labs that an investigation was launched. After her death, Lacks’ family remained unaware that her cells were being used in labs around the world and fueling groundbreaking scientific discoveries.

Scientist 69

Scientist Research Genetics Cosmetics 69

Roots Analysis

SEPTEMBER 18, 2023

Owing to the ability to preserve a wide variety of elements for such a long period of time, including cells, tissues, blood and DNA, cryopreservation has emerged as a key focus area for stakeholders in the biopharmaceutical industry.

Engineer 52

Engineer Containment In-Vivo Production 52

Drug Discovery World

FEBRUARY 8, 2023

This helps ensure a high concentration of loaded capsids and adequate removal of impurities, such as host cell proteins and DNA. In AAV therapeutics, empty capsids are those that are not packaged with the therapeutic DNA. Robustness: to be able to tolerate matrix effects (type of buffer, residual contaminants, ionic strength, etc.)

Gene Therapy 52

Gene Therapy Gene Antibody Production 52

Drug Discovery World

MAY 11, 2023

In addition, their cumbersome developmental process limits their use for fast-tracked new modalities, such as DNA-based vaccines. In addition, there are other sources of protein contamination, such as from cell culture medium supplements — for example, bovine serum albumin.” coli, and Pichia cells.

Production 52

Production Gene Protein Vaccination 52

Drug Discovery World

FEBRUARY 2, 2023

The lead AdAPT candidate, AdAPT-001, in a Phase I/II clinical trial for the treatment of cancer, carries an extra piece of DNA called a transgene that binds to, traps, and neutralises a molecule called transforming growth factor-beta (TGF-b), which tumours hijack to suppress the antitumour immune response.

Manufacturing 52

Manufacturing Clinical Trials Clinical Trials Drugs 52

The Pharma Data

MAY 13, 2021

every day are contaminated with at LEAST ONE TOXIC ingredient. food is “A food in which the gene from one species has been forced into the DNA of another species and the process itself creates all sorts of unpredicted side effects.”. These ingredients can be harmful and even LIFE THREATENING. A better definition of a G.M.O.

Production 52

Production Containment Genetics Doctors 52

XTalks

SEPTEMBER 14, 2020

Unlike COVID-19 tests that need to be processed through PCR machines, the new rapid test is processed through a handheld DNA analyzer. There is also a risk of the mask becoming contaminated from viral droplets while on the face and once contaminated, the virus may get into the eyes, nose or mouth when the mask is removed.

Vaccination 92

Vaccination Vaccine Antibody Life Science 92